J.M. Wojtowicz, Ph.D.
Phone: (416) 978-2899 Fax: (416) 978-4373 Email: firstname.lastname@example.org Webpage: http://www.newneuron.com/ Address: Department of Physiology
Medical Sciences Building, Room 3214
University of Toronto
1 King's College Circle
Toronto, Ontario, Canada
ACADEMIC STATUSDepartmental Status: Full Professor & Graduate Co-Coordinator
Primary/Cross-Appointments: Department of Physiology
Degrees: Ph.D. 1978
Courses taught: Graduate - PSL 1026 Physiological experimentation, PSL 1050 Hippocampus (organizer). Undergraduate - PSL 440 Systems Neuroscience, PSL 374 Advanced Physiology Laboratory (Course Director), PSL 300 Human Physiology I .
RESEARCHResearch Divisions: Platform Head, Brain Research and Integrated Neurophysiology B.R.A.I.N. Platform
Research Interests: Mechanisms and Functions of adult neurogenesis in the mammalian brain.
Keywords: Synapse, Hippocampus, Neuronal Plasticity, Adult Neurogenesis, Learning and Memory, Mechanisms of Cognitive Aging, Alzheimer’s disease, Stroke.Detailed Description:
Mechanism of long-term neuronal plasticity in rat brain. Research is directed towards understanding of long-term changes in brain circuit organization and synaptic connections during learning. Experiments are done primarily in vivo or in brain slices with the use of immunohistochemical, electrophysiological, ultrastructural, pharmacological techniques. These are supplemented by targeted behavioural tests. Questions under investigation are: a) What are the molecular and physiological factors controlling the rate of neuronal production and growth in the adult brain? b) What is the role of the new-born neurons in the dentate gyrus ? c) What are the differences between young and old neurons co-existing within the adult brain structure? d) Is cognitive decline with aging caused by decrease in hippocampal neurogenesis. e) Can adult neurogenesis explain some of the cognitive changes seen in Alzheimer's and after stroke?
METHODS USEDCell and tissue culture: Brain slice, hippocampal slice cultures.
Procedures: Electrophysiology, immunohistochemistry, immunocytochemistry, in vitro electrophysiology, RIA, stereotaxic brain surgery, voltage clamp.
EQUIPMENT USEDConfocal microscope, culture hood, dissecting microscope, electrophysiology rig, fixed tissue sectioning system (vibratome), fluorescence microscope, fresh tissue sectioning systems, micropipette puller, pressure osmometer, rat running wheels, vibratome.
SAMPLE PUBLICATIONS AND ABSTRACTSClick on link to PubMed for original papers.
Wojtowicz, J.M. Adult Neurogenesis. From circuits to models. Behavioural Brain Res., 2012, 227, 490-496.
Wojtowicz, J.M. Neurogenesis-based regeneration and cognitive therapy. Is it feasible? In: Cognitive Neurorehabilitation, Evidence and Application, Second Edition. Eds: Stuss, D.T., Winocur, G., Robertson, I.H., 2008, chapt. 20, 348-359.
Abrous, N., Wojtowicz, J.M. Neurogenesis and the hippocampal memory system. In: Adult Neurogenesis. Eds: Gage, F.H., Kempermann,G., Song, H., 2008, chapt. 21, 445-465.
Wojtowicz, J.M. Adult Neurogenesis in the Hippocampus: Lessons from Natural Populations. In: Neurogenesis in the Adult Brain I. Eds. Seki,T., Sawamoto,K., Parent , J.M., Alvarez-Buylla,A., 2011, chapt. 10, Springer.
Adam Mosa - MSc. Candidate
Charles Jian - PSL 498 project student
Johnny Huang - PSL 498 project student
Fabiha Rahman, Mable Chan, and Maria Sherman - ROP 299 project students
PRESENT COLLABORATIONSWithin the Department of Physiology:
Dr. Beverly Orser
Dr. Rudy Boonstra
Outside the Department of Physiology:
Dr. Sue Becker - Psychology/McMaster/Canada
Dr. Gordon Winocur - Psychology/Baycrest/Canada
Dr. David Smith - Psychology/Cornell/US
Dr. David Cechetto - Anatomy & Cell Biology/UWO/Canada
Committee member/Officer of national or international scientific organization: Member - Society for Neuroscience, Canadian Association of Neuroscience, Canadian Physiological Society, Southern Ontario Neuroscience Association